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J Liver Cancer > Volume 16(1); 2016 > Article
Journal of Liver Cancer 2016;16(1):31-37.
DOI: https://doi.org/10.17998/jlc.16.1.31    Published online March 31, 2016.
Confirmation of HIF-1α Independent Pathway in the Progression of HepG2 Cells by Hypoxic Condition
Sang Woo Lee1,2, Jae Uk Chong1, Seon Ok Min1,2, Kyung Sik Kim1,2,3
1Department of Surgery, Yonsei University College of Medicine
2Graduate Program of Nano Science and Technology, Yonsei University Graduate School
3Cell Therapy Center, Severance Hospital, Seoul, Korea
Copyright ©2016 by The Korean Liver Cancer Association
Abstract
Background/Aims
When hepatocellular carcinoma (HCC) is exposed to hypoxic condition, HIF-1α is activated and results in angiogenesis and increased tumor burden. Although inhibition of HIF-1α may reduce tumor growth, there are some limitations to control tumor growth completely. For a more effective therapy for HCC, we investigated HIF-1α independent pathway related tumor growth with angiogenesis.
Methods
We cultured HepG2 cells (HCC cell line) in both normoxia and hypoxia conditions. These cells were divided into three groups: a echinomycin treated group, a echinomycin and quinazoline treated group and a control group without any treatments. Growth morphologies of cells were observed with a microscope after 24 hours. Immunocytochemistry assay was done to detect the angiogenesis during inhibition of HIF-1α and/or NF-κB in hypoxia condition, and compared with results in normoxia condition.
Results
In normoxia, the expression of HIF-1α on tumor growth was not found. In hypoxia, inhibition of HIF-1α reduced the tumor growth compared to the control group. But, inhibition of both HIF-1α and NF-κB did not show apparent reduction of tumor growth as shown in HIF- 1α only group.
Conclusions
Signaling pathways related to cancer cell growth exist through a vast network. Inhibition of one target molecule may result in over-expression of other molecules related to the tumor growth. For an effective therapy in blocking of the tumor growth, more comprehensive understanding of the network related to signaling pathways on tumor growth is necessary. (J Liver Cancer 2016;16:31-37)
Key Words: Hepatocellular carcinoma; Hep G2; Hypoxia-indcible factor 1; Angiogenesis Effect; Cancer
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